This product is highly sensitive point-of-care biological detection system using Europium particle. This detection system is able to amplify the detection limit of biological marker detection with fully integrated, self performing one step rapid testing without requiring precise temperature regulation, sophisticated hardware, or highly trained personnel. A field-deployable and ultra-sensitive test reader is developed for the quantitative measurement of biological marker via the immunochromatographic europium nano particle assay. This reader is portable and very simple to use. This system is widely applicable to the diagnosis of infectious diseases and diagnosis of valuable biological markers which require higher sensitivity than current diagnostic tool.